Analysis of Cytokines in Sera from Type 1 Diabetic Patients at Diagnosis.

Yoon,  In Suk; Shin,  Choong Ho; Yang,  Sei Won

doi:2011.16.1.13

Ann Pediatr Endocrinol Metab > Volume 16(1); 2011 > Article

Original Article

Journal of Korean Society of Pediatric Endocrinology 2011;16(1): 13-19.

DOI: https://doi.org/10.6065/jkspe.2011.16.1.13

Analysis of Cytokines in Sera from Type 1 Diabetic Patients at Diagnosis.

In Suk Yoon, Choong Ho Shin, Sei Won Yang

¹Department of Pediatrics, Gundan Top Hospital, Incheon, Korea.
²Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea. growth@snu.ac.kr

Abstract

PURPOSE
Diverse cytokines influence the pathogenesis of type 1 diabetes mellitus (T1DM) in different ways. We studied the profile of cytokines in sera from type 1 diabetic patients at diagnosis. METHODS: Serum levels of 11 cytokines (IL-1alpha, IL-1beta, IL-1Ra, IL-2, IL-4, IL-6, IL-10, IL-12 (p70), INF-gamma, and TNF-alpha) from 38 newly-diagnosed T1DM patents and 39 healthy controls were measured, using multiplex immunoanalytic xMAP.
RESULTS
Patients showed significantly higher levels of IL-1beta (P < 0.01), IL-10 (P < 0.01), and TNF-alpha (P = 0.019), than the healthy controls. In 12 of 35 patients, the insulin autoantibody (IAA) was positive (34%) and the level of IAA was correlated with IL-10 (r = 0.454, P = 0.006), and TNF-alpha (r = 0.368, P = 0.030).
CONCLUSION
These results suggest that IL-1beta, TNF-alpha, and IL-10 play a role in the pathogenesis of T1DM, and the level of the IAA is correlated with IL-10 and TNF-alpha.

Keywords: Diabetes mellitus, type 1;Cytokines;Interleukin-1beta;Tumor necrosis factor-alpha;Interleukin-10;Islet cell antibody