The review article [1], "Long-read next-generation sequencing for molecular diagnosis of pediatric endocrine disorders," provides a comprehensive overview of the benefits of long-read next-generation sequencing (NGS) in the detecting structural variants, repeat expansions, and its applications in difficult-to-sequence regions. This review particularly highlights the importance of haplotype phasing in understanding the genetic underpinnings of autosomal recessive diseases and the parental origin of de novo mutations and emphasizes the role of long-read NGS for enhancing haplotype phasing. For haplotype phasing, binary alignment map (BAM) format from FASTQ files of NGS are analyzed using various methods including HapCUT2, WhatsHap, and SAMTools. Notably, SAMTools is a well-known software tool for processing short-read NGS data for reading, writing, editing, indexing, viewing and phasing BAM formats [2]. However, short-read NGS has limitations due to the short-read length of 100–200 bp, which making it difficult to provide linkage information between distantly spaced single nucleotide polymorphisms. Additionally, it faces challenges with read errors and accurately analyzing complex structural variants and repetitive regions.